dnaN

168

DNA polymerase III (beta subunit), beta clamp, part of the replisome, also involved in DNA mismatch repair, inhibitor of DnaA oligomerization

Locus: BSU_00020

Molecular weight: 41.94 kDa

Isoelectric point: 4.72

Protein length: 378 aa

Gene length: 1137 bp

Function: DNA replication, DNA repair

Product: DNA polymerase  III (beta subunit), beta clamp

Essential: yes

E.C.: 2.7.7.7

Synonyms: dnaN, dnaG

Genomic Context

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List of homologs in different organisms, belongs to COG0592 (Galperin et al., 2021)

Gene

Coordinates: 1,939  3,075

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• DnaN overexpression or release from the replisome decreases association of YabA with oriC, increases association of DnaA with oriC PubMed
• recruits MutS to the site of DNA replication (replisome) PubMed
• required for bacteriophage SPP1 replication PubMed
• inhibits oligomerization and helix formation of DnaA PubMed

Protein family

• Beta sliding clamp family (single member, according to UniProt)

Structure

• 4TR6 (PDB) PubMed
• 6E8D (PDB) (complex with MutL) PubMed
• P05649 (AlphaFold)

Localization

• nucleoid (mid-cell spot) PubMed PubMed
• forms large assemblies on DNA, called clamp zones PubMed
• forms foci PubMed

Expression and Regulation

Operons

• Genes: dnaA-dnaN

  Description: PubMed

  Regulation

  • negatively controlled by DnaA PubMed and Spo0A PubMed

  Regulatory mechanism

  • Spo0A: repression, PubMed, in spo0A regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of dnaA PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

GFP fusion

• PubMed  PubMed

References

Reviews

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Original Publications

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Labs working on this gene/protein

• Philippe Noirot, Jouy-en-Josas, France homepage