rpoC

168

RNA polymerase beta' subunit

Locus: BSU_01080

Molecular weight: 133.92 kDa

Isoelectric point: 8.86

Protein length: 1199 aa

Gene length: 3600 bp

Function: transcription

Product: RNA polymerase beta' subunit

Essential: yes

E.C.: 2.7.7.6

Synonyms: rpoC

Genomic Context

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List of homologs in different organisms, belongs to COG0086 (Galperin et al., 2021)

Gene

Coordinates: 125,562 → 129,161

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate + RNA_(n) --> diphosphate + RNA_{(n + 1)} (according to UniProt)

Protein family

• RNA polymerase beta' chain family (single member, according to UniProt)

Structure

• 6WVJ (PDB) (RNA polymerase elongation complex, RpoA-RpoB-RpoC-YloH) PubMed
• 3IYD (PDB) (RNA polymerase from E. coli, 50% identity) PubMed
• 8EHQ (PDB) (the paused transcription complex of Mycobacterium tuberculosis RNA polymerase with B. subtilis NusG) PubMed
• 8EOE (PDB) (the elongation complex of Mycobacterium tuberculosis RNA polymerase with B. subtilis NusG) PubMed
• P37871 (AlphaFold)

Modification

• phosphorylated on Arg-335 and Arg-803 PubMed

Localization

• colocalizes with the nucleoid PubMed

Expression and Regulation

Operons

• Genes: rpoB-rpoC

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed
  • the leader mRNA is processed upstream of rpoB by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

Reviews

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Original Publications

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