rpoB

168

RNA polymerase beta subunit

Locus: BSU_01070

Molecular weight: 133.38 kDa

Isoelectric point: 4.73

Protein length: 1193 aa

Gene length: 3582 bp

Function: transcription

Product: RNA polymerase beta subunit

Essential: yes

E.C.: 2.7.7.6

Synonyms: rpoB

Genomic Context

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List of homologs in different organisms, belongs to COG0085 (Galperin et al., 2021)

Gene

Coordinates: 121,919 → 125,500

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Nucleoside triphosphate + RNA_(n) --> diphosphate + RNA_{(n + 1)} (according to UniProt)

Protein family

• RNA polymerase beta chain family (single member, according to UniProt)

Structure

• 6WVJ (PDB) (RNA polymerase elongation complex, RpoA-RpoB-RpoC-YloH) PubMed
• 3IYD (PDB) (RNA polymerase from E. coli, 59% identity) PubMed
• 3MLQ (PDB) (RNA polymerase interacting domain of Thermus thermophilus Mfd with the Thermus aquaticus RpoB beta1 domain) PubMed
• 8EHQ (PDB) (the paused transcription complex of Mycobacterium tuberculosis RNA polymerase with B. subtilis NusG) PubMed
• 8EOE (PDB) (the elongation complex of Mycobacterium tuberculosis RNA polymerase with B. subtilis NusG) PubMed
• P37870 (AlphaFold)

Modification

• phosphorylated on (Arg-312 OR Arg-313), Arg-539, (Arg-693 OR Arg-694), Arg-827, and Arg-1106 PubMed

Localization

• cytoplasm

Expression and Regulation

Operons

• Genes: rpoB-rpoC

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed
  • the leader mRNA is processed upstream of rpoB by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

FLAG-tag construct

• GP2043 rpoB-3xFLAG ermC (based on pGP1087), available in Jörg Stülke's lab

LacZ fusion

• pGP2683 (cat) based on pAC5, available in Jörg Stülke's lab
• pGP2682 (aphA3) based on pAC7, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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