spoIISA

168

lethal when synthesized during vegetative growth in the absence of SpoIISB

Locus: BSU_12830

Molecular weight: 28.91 kDa

Isoelectric point: 4.68

Protein length: 248 aa

Gene length: 747 bp

Function: programmed cell death

Product: toxin

Essential: no

Synonyms: spoIISA, ykaC

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 1,348,612 → 1,349,358

The protein

Protein family

• SpoIISA toxin family (single member, according to UniProt)

Domains

• three putative membrane-spanning segments and a cytoplasmic domain

Structure

• 3O6Q (PDB) (cytoplasmic fragment of SpoIISA (CSpoIISA) in complex with SpoIISB) PubMed
• O34853 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: spoIISA-spoIISB

  Description: PubMed

  Sigma factors

  • SigK: sigma factor, in sigK regulon

Biological materials

Mutant

• MGNA-A006 (spoIISA::erm), available at the NBRP B. subtilis, Japan
• 1S123 ( spoIISA::kan), PubMed, available at BGSC
• BKE12830 (ΔspoIISA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGAATCCCTCACAT,  downstream forward: _UP4_ATTGAGGAGGAAGGTGAAGG
• BKK12830 (ΔspoIISA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTCGAATCCCTCACAT,  downstream forward: _UP4_ATTGAGGAGGAAGGTGAAGG

References

Reviews

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Original Publications

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