scpA

168

part of the condensin complex, chromosomal origin condensation and segregation

Locus: BSU_23220

Molecular weight: 29.46 kDa

Isoelectric point: 4.79

Protein length: 251 aa

Gene length: 756 bp

Function: segregation of replication origins

Product: DNA segregation and condensation protein

Essential: yes

Synonyms: scpA, ypuG

Genomic Context

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List of homologs in different organisms, belongs to COG1354 (Galperin et al., 2021)

Gene

Coordinates: 2,425,831  2,426,586

Phenotypes of a mutant

• essential PubMed
• ''scpA'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth PubMed

The protein

Protein family

• scpA family (single member, according to UniProt)

Structure

• 3ZGX (PDB) (complex between the head domain of Smc (aa 1 - 219 and 983 - 1186) and the N-terminal winged-helix domain of ScpA) PubMed
• 4I98 (PDB) (complex between ScpA (aa 1-160)-ScpB (aa 1-183) PubMed
• P35154 (AlphaFold)

Localization

• nucleoid (Multiple) PubMed
• the condensin (Smc)₂-ScpA-ScpB complex forms two bipolar assemblies on the chromosome, one in each cell half, localization depends on ParA PubMed
• the condensin (Smc)2-ScpA-ScpB complex is loaded by ParB to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute PubMed

Expression and Regulation

Operons

• Genes: scpA-scpB-ypuI

  Description: PubMed

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

Biological materials

Mutant

• MGNA-A104 (ypuG::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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Original Publications

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