sunA

168

sublancin 168 is post-translationally modified antimicrobial precursor peptide of the glycocin class

Locus: BSU_21480

Molecular weight: 5.85 kDa

Isoelectric point: 7.96

Protein length: 56 aa

Gene length: 171 bp

Function: exported antimicrobial peptide

Product: sublancin 168 antimicrobial precursor peptide

Essential: no

Synonyms: sunA, yolG

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,269,521  2,269,691

The protein

Structure

• 2MIJ (PDB) PubMed
• P68577 (AlphaFold)

Modification

• contains a glucose attached to a cysteine residue, glycosylation is essential for its antimicrobial activity PubMed

Localization

• secreted PubMed

Expression and Regulation

Operons

• Genes: sunA-sunT-bdbA-sunS-bdbB

  Description: PubMed

  Regulation

  • repressed by Rok-DnaA PubMed
  • expression starts in the stationary phase PubMed
  • expression is heterogeneous in the population, this is mediated by AbrB, Rok, and Spo0A PubMed

  Regulatory mechanism

  • Rok: repression, PubMed, in rok regulon
  • Abh: activation, PubMed, in abh regulon
  • DnaA: repression, Rok-DnaA PubMed, in dnaA regulon
  • YvrHb: activation, PubMed, in yvrHb regulon
  • AbrB: repression, PubMed, in abrB regulon

  Additional information

  • the mRNA is very stable (> 15 min) PubMed
  • the amount of the mRNA is substantially decreased upon depletion of RNase Y PubMed

• Genes: sunA

  Description: PubMed

  Regulation

  • repressed by Rok-DnaA PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• GP1563 (aphA3), available in Jörg Stülke's labb
• GP1565 (sunA-sunI, aphA3), available in Jörg Stülke's lab
• BKE21480 (sunA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTAAAACCTCCCCAT,  downstream forward: _UP4_TAAAACATTTGTAGAGGGAA
• BKK21480 (sunA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTAAAACCTCCCCAT,  downstream forward: _UP4_TAAAACATTTGTAGAGGGAA

References

Reviews

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Original Publications

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