epsA

168

extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation

Locus: BSU_34370

Molecular weight: 25.75 kDa

Isoelectric point: 6.07

Protein length: 234 aa

Gene length: 705 bp

Function: biofilm formation

Product: unknown

Essential: no

Synonyms: epsA, yveK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3944 (Galperin et al., 2021)

Gene

Coordinates: 3,529,151 → 3,529,855

The protein

Protein family

• CapA family (with CapA and TkmA, according to UniProt)
• CpsC/CapA family (with TkmA, according to UniProt)

Structure

• P71050 (AlphaFold)

Paralogous protein(s)

• TkmA

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO

  Description: PubMed

  Regulation

  • repressed by SinR PubMed

  Regulatory mechanism

  • RemA: activation, PubMed, in remA regulon
  • AbrB: repression, PubMed, in abrB regulon
  • SinR: anti-activation, (prevents binding of RemA) PubMed, in sinR regulon
  • EAR riboswitch: processive antitermination, in EAR riboswitch

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed

Biological materials

Mutant

• MGNA-A073 (yveK::erm), available at the NBRP B. subtilis, Japan
• GP1517 (aphA3) PubMed, available in Jörg Stülke's lab
• GP1519 (''epsA-epsB'', aphA3) PubMed, available in Jörg Stülke's lab
• GP1567 ''epsA::aphA3 tkmA''::spc PubMed, available in Jörg Stülke's lab
• BKE34370 (ΔepsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATTCATAGCCTTCAG,  downstream forward: _UP4_AAACATTTCGGGGAGTGAAG
• BKK34370 (ΔepsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTATTCATAGCCTTCAG,  downstream forward: _UP4_AAACATTTCGGGGAGTGAAG

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) PubMed, available in Jörg Stülke's lab

FLAG-tag construct

• GP1526 epsA-FLAG 3x spc (based on pGP1331) PubMed, available in Jörg Stülke's lab

Antibody

• **

GFP fusion

• GP1569 epsA-gfp (spc), available in Jörg Stülke's lab

Labs working on this gene/protein

• Richard Losick, Harvard Univ., Cambridge, USA homepage

References

Reviews

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Original Publications

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