xynA

168

GH11 family endo-1,4-beta-xylanase

Locus: BSU_18840

Molecular weight: 23.20 kDa

Isoelectric point: 9.64

Protein length: 213 aa

Gene length: 642 bp

Function: xylan degradation

Product: endo-1,4-beta-xylanase

Essential: no

E.C.: 3.2.1.8

Synonyms: xynA

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,054,599 → 2,055,240

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans resulting in the release of xylobiose and xylotriose PubMed

Protein family

• glycosyl hydrolase 11 (cellulase G) family (single member, according to UniProt)

Domains

• GH 11 domain (aa 29-213) (according to UniProt)

Structure

• 2QZ3 (PDB) (complex with xylotetraose),  2Z79 (PDB), 3EXU (PDB)
• P18429 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: xynA

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'pps' and 'xynA' PubMed

Biological materials

Mutant

• BKE18840 (ΔxynA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTTACCTCCTATAAT,  downstream forward: _UP4_TAACAGATCATCCTTAATCA
• BKK18840 (ΔxynA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATGTTACCTCCTATAAT,  downstream forward: _UP4_TAACAGATCATCCTTAATCA

References

Reviews

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Original Publications

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