spsK

spsK
168

dTDP-4-dehydrorhamnose reductase

Locus
BSU_37820
Molecular weight
31.43 kDa
Isoelectric point
6.08
Protein length
Gene length
Function
rhamnose biosynthesis, spore crust polysaccharide synthesis
Product
dTDP-4-dehydrorhamnose reductase
Essential
no
E.C.
1.1.1.133
Synonyms
spsK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1091 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,883,427  3,884,278
Phenotypes of a mutant
the mutant tends to acquire suppressor mutations that result in improved growth PubMed
production of spores lacking a visible crust PubMed
The protein
Protein family
dTDP-4-dehydrorhamnose reductase family (single member, according to UniProt)
Structure
3SC6 (PDB) (dTDP-4-dehydrorhamnose reductase (rfbD) from Bacillus anthracis str. Ames in complex with NADP; 42% identity, 72% similarity)
Expression and Regulation
Operons
Description
Regulation
expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed
Regulatory mechanism
GerE: activation, PubMed, in gerE regulon
Sigma factors
SigE: sigma factor, PubMed, in sigE regulon
SigK: sigma factor, PubMed, in sigK regulon
Additional information
the mRNA is very stable (half-life > 15 min) PubMed
Open in new tab

spsAspsL

2025-04-07 10:32:08

ghost

135

8fd40e70daa9bc3b7d469fa252e122a98e4555de

9FCD794B68194823BCA66D7E89CC93E5AF9CC0AA

Biological materials
Mutant
BKE37820 (spsK::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TACCTTTGTCAATCAGATCA,  downstream forward: _UP4_TATTTGCAGGAAAGGAGCAG
BKK37820 (spsK::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_TACCTTTGTCAATCAGATCA,  downstream forward: _UP4_TATTTGCAGGAAAGGAGCAG
References
Loading

4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465

Page visits: 4039

Time of last update: 2025-04-28 07:24:57

Author of last update: Jstuelk