parC

168

subunit of DNA topoisomerase IV

Locus: BSU_18100

Molecular weight: 91.14 kDa

Isoelectric point: 6.31

Protein length: 806 aa

Gene length: 2421 bp

Function: chromosome segregation and compaction, resolution of head-on DNA replication-Transcription conflicts

Product: subunit of DNA topoisomerase IV

Essential: yes

E.C.: 5.99.1.-

Synonyms: parC, grlA

Genomic Context

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List of homologs in different organisms, belongs to COG0188 (Galperin et al., 2021)

Gene

Coordinates: 1,935,448  1,937,868

Phenotypes of a mutant

• essential PubMed
• a parC point mutation has been isolated upon selction at low pressure PubMed
• upon overexpression, topoisomerase IV (ParE-ParC) can take over the functions of the type 1A topoisomerases TopA and TopB PubMed

The protein

Catalyzed reaction/ biological activity

• ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)
• binds to abasic DNA lesions PubMed

Protein family

• Topoisomerase IIA GyrA/ParC subunit family (together with GyrA) (according to UniProt)

Structure

• 4I3H (PDB) (the ParE-ParC complex from Streptococcus pneumoniae, 63% identity) PubMed
• 1WP5 (PDB) (C-terminal domain, Geobacillus stearothermophilus)
• Q45066 (AlphaFold)

Paralogous protein(s)

• GyrA

Localization

• cytoplasm (Homogeneous) PubMed
• polar localization in actively growing cells PubMed

Expression and Regulation

Operons

• Genes: parE-parC

  Description: PubMed

  Regulation

  • induced by DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of parE PubMed

References

Reviews

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Original Publications

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