menE

168

o-succinylbenzoate-CoA ligase

Locus: BSU_30790

Molecular weight: 54.02 kDa

Isoelectric point: 5.47

Protein length: 486 aa

Gene length: 1461 bp

Function: biosynthesis of menaquinone

Product: o-succinylbenzoate-CoA ligase

Essential: yes

E.C.: 6.2.1.26

Synonyms: menE

Genomic Context

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List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

Gene

Coordinates: 3,147,350  3,148,810

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + 2-succinylbenzoate + CoA --> AMP + diphosphate + 2-succinylbenzoyl-CoA PubMed

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Structure

• 5BUQ (PDB) PubMed
• 5X8F (PDB) (complex with AMP and product analog) PubMed
• 5X8G (PDB) (complex with product analog) PubMed
• P23971 (AlphaFold)

Modification

• MenE is acetylated on Lys-471 by AcuA, acetylation results in inactivation of MenE PubMed
• deacetylation is catalyzed by SrtN PubMed

Expression and Regulation

Operons

• Genes: menE-menC

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

  Additional information

  • A ncRNA is predicted between 'mntA' and 'menC' PubMed

• Genes: menB-menE-menC

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'mntA' and 'menC' PubMed

• Genes: menF-menD-ytxM-menB-menE-menC

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'mntA' and 'menC' PubMed

References

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