rnpA

168

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs

Locus: BSU_41050

Molecular weight: 13.66 kDa

Isoelectric point: 10.8

Protein length: 116 aa

Gene length: 351 bp

Function: cleavage of precursor sequences from the 5 ends of pre-tRNAs

Product: protein component of RNase P (substrate specificity)

Essential: yes

E.C.: 3.1.26.5

Synonyms: rnpA

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Information processing, RNA synthesis and degradation, RNases, Endoribonucleases

Information processing, Protein synthesis, modification and degradation, Translation, tRNA modification and maturation

Groups of genes, Essential genes

List of homologs in different organisms, belongs to COG0594 (Galperin et al., 2021)

Gene

Coordinates: 4,214,753  4,215,103

Phenotypes of a mutant

essential PubMed

The protein

Catalyzed reaction/ biological activity

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to UniProt)
5' end maturation of tmRNA PubMed

Protein family

RnpA family (single member, according to UniProt)

Domains

RNR motif: residues 60-68, stabilizes complexes with both RNA part of RNase P (''rnpB'') and with pre-trnA-Ile (rnpB-RnpA-pre-trnA-Ile) PubMed

Structure

1A6F (PDB)  PubMed
3Q1R (PDB) (complex of RnpA with RNA RnpB and trnA-Ile, from Thermotoga maritima) PubMed
P25814 (AlphaFold)

Kinetic information

K_D (RnpA-RnpB interaction): 40 nM PubMed

Expression and Regulation

Operons

Genes: rpmH-rnpA-yidC1-jag

Description: PubMed

Regulation

expression is reduced in motile cells as compared to non-motile cells PubMed

Biological materials

Two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

Carol Fierke, University of Michigan, Ann Arbor, USA homepage
Roland Hartmann, Marburg University, Germany homepage

References

Reviews

Loading

Original Publications

Loading