rnpA

168

protein component of RNase P, metal-dependent 5 end maturation of precursor tRNAs

Locus: BSU_41050

Molecular weight: 13.66 kDa

Isoelectric point: 10.8

Protein length: 116 aa

Gene length: 351 bp

Function: cleavage of precursor sequences from the 5 ends of pre-tRNAs

Product: protein component of RNase P (substrate specificity)

Essential: yes

E.C.: 3.1.26.5

Synonyms: rnpA

Genomic Context

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List of homologs in different organisms, belongs to COG0594 (Galperin et al., 2021)

Gene

Coordinates: 4,214,753  4,215,103

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor (according to UniProt)
• 5' end maturation of tmRNA PubMed

Protein family

• RnpA family (single member, according to UniProt)

Domains

• RNR motif: residues 60-68, stabilizes complexes with both RNA part of RNase P (''rnpB'') and with pre-trnA-Ile (rnpB-RnpA-pre-trnA-Ile) PubMed

Structure

• 1A6F (PDB)  PubMed
• 3Q1R (PDB) (complex of RnpA with RNA RnpB and trnA-Ile, from Thermotoga maritima) PubMed
• P25814 (AlphaFold)

Kinetic information

• K_D (RnpA-RnpB interaction): 40 nM PubMed

Expression and Regulation

Operons

• Genes: rpmH-rnpA-yidC1-jag

  Description: PubMed

  Regulation

  • expression is reduced in motile cells as compared to non-motile cells PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Labs working on this gene/protein

• Carol Fierke, University of Michigan, Ann Arbor, USA homepage
• Roland Hartmann, Marburg University, Germany homepage

References

Reviews

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Original Publications

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