perR

168

transcriptional repressor of the peroxide regulon, sensor of the intracellular Fe/Mn ratio

Locus: BSU_08730

Molecular weight: 16.28 kDa

Isoelectric point: 5.89

Protein length: 145 aa

Gene length: 438 bp

Function: regulation of the response to peroxide

Product: transcriptional repressor (Fur family)

Essential: no

Synonyms: perR, ygaG

Genomic Context

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List of homologs in different organisms, belongs to COG0735 (Galperin et al., 2021)

Gene

Coordinates: 944,487  944,924

Phenotypes of a mutant

• resistant to hydrogen peroxide, accumulates a porphyrin-like compound, and grows very slowly (due to heme sequestration by KatA) PubMed

The protein

Protein family

• Fur family (according to UniProt)

Cofactors

• contains an Fe²⁺ at the regulatory site and Zn²⁺ PubMed

Structure

• 2RGV (PDB) 3F8N (PDB)
• P71086 (AlphaFold)

Modification

• selective metal catalyzed oxidation of two histidine residues of the regulatory site results in induction (loss of DNA-binding activity) PubMed

Effectors of protein activity

• responds to the presence of hydrogen peroxide PubMed

Paralogous protein(s)

• Zur, Fur

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: perR

  Description: PubMed

  Regulation

  • induced in the presence of hydrogen peroxide (PerR) PubMed

  Regulatory mechanism

  • PerR: repression, PubMed, in perR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: ygaF-perR

  Description: PubMed

Biological materials

Mutant

• GP3171 perR::kan trpC2 available in Jörg Stülke's lab
• MGNA-A258 (ygaG::erm), available at the NBRP B. subtilis, Japan
• HB0509 (spc), available in John Helmann's and Jörg Stülke's labs, also GP868 (fur::mls, perR::spc).
• 1A903 ( perR::kan), PubMed, available at the Bacillus Genetic Stock Center
• BKE08730 (ahpC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTCATGCACCTCTCT,  downstream forward: _UP4_TAAAAATAAGCTGACCGCAC
• BKK08730 (ahpC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCGTCATGCACCTCTCT,  downstream forward: _UP4_TAAAAATAAGCTGACCGCAC

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

Reviews

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Original Publications

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