sacB
168
levansucrase
Locus
BSU_34450
Molecular weight
52.80 kDa
Isoelectric point
6.18
Function
utilization of sucrose, production of levan
Product
levansucrase
Essential
no
E.C.
2.4.1.10
Synonyms
sacB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,536,012 3,537,433
Phenotypes of a mutant
defective in tomato root colonization in the presence of sucrose PubMed
The protein
Catalyzed reaction/ biological activity
[6)-β-D-fructofuranosyl-(2→](n) α-D-glucopyranoside + sucrose --> [6)-β-D-fructofuranosyl-(2→](n+1) α-D-glucopyranoside + D-glucose (according to UniProt)
Protein family
glycosyl hydrolase 68 family (single member, according to UniProt)
Structure
1PT2 (PDB) (complex with sucrose), 1OYG (PDB)
secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
Expression and Regulation
Operons
Biological materials
Mutant
LacZ fusion
References
Reviews
Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis.
FEMS microbiology letters. 2010 Dec; 313(1):1-9. doi:10.1111/j.1574-6968.2010.02085.x. PMID:20735481
Original Publications
Tailoring of levansucrase product size by a comparative molecular dynamics approach.Enzyme and microbial technology. 2024 Dec 23; 184:110577. PMID: 39721367
Insights into the heterogeneity of levan polymers synthesized by levansucrase Bs-SacB from Bacillus subtilis 168.Carbohydrate polymers. 2024 Jan 1; 323:121439. PMID: 37940304
Rational design of a self-assembly promoting fusion domain enhances high molecular weight levan synthesis by levansucrase SacB.International journal of biological macromolecules. 2023 Jun 15; :125442. PMID: 37330087
Sucrose triggers a novel signaling cascade promoting Bacillus subtilis rhizosphere colonization.The ISME journal. 2021 Mar 26; . PMID: 33772107
The molecular basis of the nonprocessive elongation mechanism in levansucrases.The Journal of biological chemistry. 2020 Dec 10; . PMID: 33303628
Implications of the mutation S164A on Bacillus subtilis levansucrase product specificity and insights into protein interactions acting upon levan synthesis.International journal of biological macromolecules. 2020 Oct 15; 161:898-908. PMID: 32553967
Effect of metal ions on levan synthesis efficiency and its parameters by levansucrase from Bacillus subtilis.
International journal of biological macromolecules. 2019 Jan 28; . pii:S0141-8130(18)35668-X. doi:10.1016/j.ijbiomac.2019.01.155. PMID:30703417
Understanding the transfer reaction network behind the non-processive synthesis of low molecular weight levan catalyzed by Bacillus subtilis levansucrase.
Scientific reports. 2018 Oct 09; 8(1):15035. doi:10.1038/s41598-018-32872-7. PMID:30301900
Maturation of polycistronic mRNAs by the endoribonuclease RNase Y and its associated Y-complex in .
Proceedings of the National Academy of Sciences of the United States of America. 2018 May 24; . pii:201803283. doi:10.1073/pnas.1803283115. PMID:29794222
Enzymatic synthesis and structural characterization of theanderose through transfructosylation reaction catalyzed by levansucrase from Bacillus subtilis CECT 39.
Journal of agricultural and food chemistry. 2017 Nov 13; . doi:10.1021/acs.jafc.7b03092. PMID:29131629
Co-production of Fructooligosaccharides and Levan by Levansucrase from Bacillus subtilis natto with Potential Application in the Food Industry.
Applied biochemistry and biotechnology. 2017 Sep 04; . doi:10.1007/s12010-017-2587-0. PMID:28871528
Synthesis of potentially-bioactive lactosyl-oligofructosides by a novel bi-enzymatic system using bacterial fructansucrases.
Food research international (Ottawa, Ont.). 2015 Dec; 78:258-265. pii:S0963-9969(15)30209-X. doi:10.1016/j.foodres.2015.09.035. PMID:28433290
Size product modulation by enzyme concentration reveals two distinct levan elongation mechanisms in Bacillus subtilis levansucrase.
Glycobiology. 2016 Apr; 26(4):377-85. doi:10.1093/glycob/cwv112. PMID:26646447
Intrinsic Levanase Activity of Bacillus subtilis 168 Levansucrase (SacB).
PloS one. 2015; 10(11):e0143394. doi:10.1371/journal.pone.0143394. PMID:26600431
Bacillus subtilis 168 levansucrase (SacB) activity affects average levan molecular weight.
Carbohydrate polymers. 2015 Nov 05; 132:338-44. doi:10.1016/j.carbpol.2015.06.056. pii:S0144-8617(15)00565-2. PMID:26256357
Design of chimeric levansucrases with improved transglycosylation activity.
Applied and environmental microbiology. 2012 Mar; 78(6):1820-5. doi:10.1128/AEM.07222-11. PMID:22247149
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
Journal of molecular microbiology and biotechnology. 2009; 16(1-2):53-68. doi:10.1159/000142894. PMID:18957862
Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB.
BMC microbiology. 2008 Jan 15; 8:8. doi:10.1186/1471-2180-8-8. PMID:18197985
yveB, Encoding endolevanase LevB, is part of the sacB-yveB-yveA levansucrase tricistronic operon in Bacillus subtilis.
Microbiology (Reading, England). 2001 Dec; 147(Pt 12):3413-9. . PMID:11739774
Cloning structural gene sacB, which codes for exoenzyme levansucrase of Bacillus subtilis: expression of the gene in Escherichia coli.
Journal of bacteriology. 1983 Mar; 153(3):1424-31. . PMID:6402497
Cloning and preliminary characterization of the sacS locus from Bacillus subtilis which controls the regulation of the exoenzyme levansucrase.
Molecular & general genetics : MGG. 1987 Jun; 208(1-2):114-20. . PMID:3039303
Modulation of Bacillus subtilis levansucrase gene expression by sucrose and regulation of the steady-state mRNA level by sacU and sacQ genes.
Journal of bacteriology. 1986 Oct; 168(1):380-8. . PMID:2428811
Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system.
Journal of bacteriology. 1990 Feb; 172(2):1043-50. . PMID:2105292
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Time of last update: 2025-07-11 11:26:43
Author of last update: Jstuelk