recN

168

DNA repair and genetic recombination

Locus: BSU_24240

Molecular weight: 64.31 kDa

Isoelectric point: 4.8

Protein length: 576 aa

Gene length: 1731 bp

Function: DNA repair and genetic recombination

Product: DNA integrity scanning protein

Essential: no

Synonyms: recN

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0497 (Galperin et al., 2021)

Gene

Coordinates: 2,520,557  2,522,287

Phenotypes of a mutant

• reduced survival after DNA replication arrest imposed by inhibition of PolC activity PubMed

The protein

Catalyzed reaction/ biological activity

• RecN stimulates polymerizing activity of PnpA PubMed

Protein family

• recN family (single member, according to UniProt)

Structure

• 4AD8 (PDB) (from Deinococcus radiodurans, 30% identity) PubMed
• P17894 (AlphaFold)

Localization

• nucleoid (multiple) PubMed
• evenly distributed in growing cells PubMed
• upon DNA damage, RecN forms a single focus per nucleoid PubMed, recruitment is affected by the presence of Fumarase and depends on RecA and AddA-AddB-mediated end resection PubMed

Expression and Regulation

Operons

• Genes: ahrC-recN

  Description: PubMed

  Regulation

  • by sRNA sr1

  Additional information

  • expression is fourfold increased upon depletion of ''nusA'' Reference

• Genes: dxs-tlyA-ahrC-recN

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

Biological materials

Mutant

• 1A891 ( recN::cat), PubMed, available at BGSC
• BKE24240 (recN::erm  trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAACAGCATTACACCTCTT,  downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA
• BKK24240 (recN::kan  trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_CAAACAGCATTACACCTCTT,  downstream forward: _UP4_TAAGCTGCGCGAGAAGCGCA

Labs working on this gene/protein

• Peter Graumann, Freiburg University, Germany homepage

References

Reviews

Loading

Original Publications

Loading