hbs

168

non-specific DNA-binding protein Hbsu

Locus: BSU_22790

Molecular weight: 9.75 kDa

Isoelectric point: 9.5

Protein length: 92 aa

Gene length: 279 bp

Function: DNA packaging, regulation of nucleoid compaction, required for the initiation of DNA replication

Product: non-specific DNA-binding protein Hbsu

Essential: yes

Synonyms: hbs, dbpA, hupA

Genomic Context

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List of homologs in different organisms, belongs to COG0776 (Galperin et al., 2021)

Gene

Coordinates: 2,385,543 → 2,385,821

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds to curved DNA without apparent sequence specificity PubMed
• binds to abasic DNA lesions PubMed
• condenses the bacterial chromosome PubMed
• Hbs-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
• plays an essential role in the initiation of DNA replication, likely acting to promote origin melting by DnaA, but also has a second DnaA-independent essential function PubMed

Protein family

• bacterial histone-like protein family (with YonN, according to UniProt)

Structure

• 1HUU (PDB) (Geobacillus stearothermophilus) PubMed
• P08821 (AlphaFold)

Modification

• phosphorylated on Arg-61 PubMed
• phosphorylation on Thr-4 PubMed
• can be acetylated on Lys-3, Lys-18, Lys-37, Lys-41, Lys-75, Lys-80, and Lys-86 PubMed
• acetylation by YfmK on Lys-80 inhibits DNA binding and nocleoid compaction PubMed
• may be acetylated by YdgE on Lys-41 and Lys-86 PubMed

Effectors of protein activity

• acetylation by YfmK on Lys-80 inhibits DNA binding and nocleoid compaction PubMed

Paralogous protein(s)

• YonN

Localization

• uniformly associated with the nucleoid in vegetative cells and forespores PubMed

Expression and Regulation

Operons

• Genes: hbs

  Description: PubMed

  Regulation

  • induced by DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigH: sigma factor, PubMed, in sigH regulon

  Additional information

  • mRNA degradation is initiated by RNase Y PubMed
  • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

Labs working on this gene/protein

• Ciaran Condon, IBPC, Paris, France Homepage
• David Dubnau,Rutgers University, Newark, USA

References

Reviews

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Original Publications

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