dnaG

dnaG
168

DNA primase, part of the replisome

Locus
BSU_25210
Molecular weight
68.62 kDa
Isoelectric point
6.71
Protein length
Gene length
Product
DNA primase
Essential
yes
E.C.
2.7.7.-
Synonyms
dnaG

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0358 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,601,528 → 2,603,339
Phenotypes of a mutant
essential PubMed
The protein
Catalyzed reaction/ biological activity
synthesis of RNA primers that initiate leading and lagging strand DNA synthesis
required for bacteriophage SPP1 replication PubMed
Protein family
DNA primase family (single member, according to UniProt)
TOPRIM domain (aa 262-343) PubMed
Structure
1D0Q (PDB) (zinc binding domain, Geobacillus stearothermophilus)
1Z8S (PDB) (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
Effectors of protein activity
the enzymatic activity is inhibited by (p)ppGpp during the stringent response, (p)ppGpp prevents DnaG from binding to replication forks and strongly inhibits primer extension PubMed
DnaG primase activity is stimulated by DnaC PubMed
Cytoplasm (Homogeneous) PubMed
Expression and Regulation
Operons
Description
Regulation
enzymatic activity is inhibited by (p)ppGpp during the 'stringent response
Regulatory mechanism
Spo0A: repression, PubMed, in spo0A regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigH: sigma factor, PubMed, in sigH regulon
Additional information
An antisense RNA is predicted for sigA PubMed
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yqxDsigA

2025-01-21 06:42:20

TPed

186

345BE3439F4414F1DA2FD49B273925B808601AB7

E9F2D1769ACB2AF65C67D8854D4F58BC49368B55

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Time of last update: 2025-01-23 10:22:53

Author of last update: Jstuelk