ruvB

168

Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase and of the RuvA-RuvB-RecU resolvasome

Locus: BSU_27730

Molecular weight: 37.31 kDa

Isoelectric point: 5.15

Protein length: 334 aa

Gene length: 1005 bp

Function: DNA repair/ recombination

Product: Holliday junction DNA helicase

Essential: no

Synonyms: ruvB

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Information processing, Genetics, DNA repair/ recombination

Information processing, Genetics, DNA repair/ recombination, Double strand breaks repair

List of homologs in different organisms, belongs to COG2255 (Galperin et al., 2021)

This gene is a member of the following regulons

LexA regulon

Gene

Coordinates: 2,835,150  2,836,154

Phenotypes of a mutant

a ruvA-ruvB recG mutant is not viable PubMed
the inactivations of ''recU and ruvA-ruvB'' are synthetically lethal PubMed
the inactivations of ''recD2 and ruvA-ruvB'' are synthetically lethal  PubMed

The protein

Catalyzed reaction/ biological activity

RuvB-ATP stimulates RecU-mediated Holliday junction resolution PubMed
ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

ruvB family (single member, according to UniProt)

Structure

1IN4 (PDB) (from Thermotoga maritima, 57% identity) PubMed
O32055 (AlphaFold)

Effectors of protein activity

DisA PubMed

Interactions

part of the RuvA-RuvB-RecU resolvasome PubMed

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: ruvA-ruvB-yrzS-queA-tgt-yrbF

Description: PubMed

Regulation

the mRNA is processed between tgt and yrbF by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Genes: ruvA-ruvB

Description: PubMed

Regulation

induced upon DNA damage (LexA) PubMed

Regulatory mechanism

LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

GP3530 (Δ(ruvA-ruvB)::kan), available in Jörg Stülke's lab
1A896 (no resistance), PubMed, available at BGSC
BKE27730 (ruvB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGCCGTTCATCCATGAACA,  downstream forward: _UP4_CATTTCCAAATGGAGGCTCC
BKK27730 (ruvB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGCCGTTCATCCATGAACA,  downstream forward: _UP4_CATTTCCAAATGGAGGCTCC

References

Reviews

Loading

Original Publications

Loading