ruvB

168

Holliday junction DNA helicase, part of the RuvA-RuvB branch migration translocase and of the RuvA-RuvB-RecU resolvasome

Locus: BSU_27730

Molecular weight: 37.31 kDa

Isoelectric point: 5.15

Protein length: 334 aa

Gene length: 1005 bp

Function: DNA repair/ recombination

Product: Holliday junction DNA helicase

Essential: no

Synonyms: ruvB

Genomic Context

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List of homologs in different organisms, belongs to COG2255 (Galperin et al., 2021)

Gene

Coordinates: 2,835,150  2,836,154

Phenotypes of a mutant

• a ruvA-ruvB recG mutant is not viable PubMed
• the inactivations of ''recU and ruvA-ruvB'' are synthetically lethal PubMed
• the inactivations of ''recD2 and ruvA-ruvB'' are synthetically lethal  PubMed

The protein

Catalyzed reaction/ biological activity

• RuvB-ATP stimulates RecU-mediated Holliday junction resolution PubMed
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• ruvB family (single member, according to UniProt)

Structure

• 1IN4 (PDB) (from Thermotoga maritima, 57% identity) PubMed
• O32055 (AlphaFold)

Effectors of protein activity

• DisA PubMed

Interactions

• part of the RuvA-RuvB-RecU resolvasome PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ruvA-ruvB-yrzS-queA-tgt-yrbF

  Description: PubMed

  Regulation

  • the mRNA is processed between tgt and yrbF by RNase Y, this requires the RicA-RicF-RicT complex PubMed

• Genes: ruvA-ruvB

  Description: PubMed

  Regulation

  • induced upon DNA damage (LexA) PubMed

  Regulatory mechanism

  • LexA: repression, PubMed, in lexA regulon

Biological materials

Mutant

• GP3530 (Δ(ruvA-ruvB)::kan), available in Jörg Stülke's lab
• 1A896 (no resistance), PubMed, available at BGSC
• BKE27730 (ruvB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGCCGTTCATCCATGAACA,  downstream forward: _UP4_CATTTCCAAATGGAGGCTCC
• BKK27730 (ruvB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGCCGTTCATCCATGAACA,  downstream forward: _UP4_CATTTCCAAATGGAGGCTCC

References

Reviews

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Original Publications

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