flgM

168

anti-SigD, regulation of flagellin, Motility and chemotaxis

Locus: BSU_35430

Molecular weight: 9.86 kDa

Isoelectric point: 9.92

Protein length: 88 aa

Gene length: 267 bp

Function: control of SigD activity

Product: anti-SigD

Essential: no

Synonyms: flgM

Genomic Context

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List of homologs in different organisms, belongs to COG2747 (Galperin et al., 2021)

Gene

Coordinates: 3,640,285 → 3,640,551

Phenotypes of a mutant

• defective in Swarming motility PubMed

The protein

Protein family

• flgM family (single member, according to UniProt)

Structure

• 1RP3 (PDB) (SigD-FlgM complex from Aqufex aeolicus, 34% identity) PubMed
• P39809 (AlphaFold)

Localization

• secreted and extracellularly degraded by the proteases Epr and WprA PubMed
• secretion of FlgM requires the FliF/FliG basal body proteins, the flagellar type III export apparatus components FliO, FliP, FliQ, FliR, FlhA, and FlhB, and the substrate specificity switch regulator FliK PubMed

Expression and Regulation

Operons

• Genes: comFA-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA

  Description: PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon
  • DegU: activation, DegU-P, PubMed, in degU regulon
  • ScoC: repression, PubMed, in scoC regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: flgM-flgN-flgK-flgL

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

• Genes: comFB-comFB-comFC-yvyF-flgM-flgN-flgK-flgL-yviE-fliW-csrA

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: yvyF-flgM-flgN-flgK-flgL

  Description: PubMed

Biological materials

Mutant

• 1A764 (no resistance), PubMed, available at BGSC
• BKE35430 (ΔflgM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT,  downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA
• BKK35430 (ΔflgM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGGATTCCTCTCGCTTT,  downstream forward: _UP4_AAGCAATAAAAAAGGAGAAA

References

Reviews

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Original Publications

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