tatAD

168

component of the TatAD-TatCD twin-arginine translocase

Locus: BSU_02630

Molecular weight: 7.37 kDa

Isoelectric point: 9.69

Protein length: 70 aa

Gene length: 213 bp

Function: TAT protein secretion

Product: component of the twin-arginine translocation pathway

Essential: no

Synonyms: tatAD, yczB

Genomic Context

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List of homologs in different organisms, belongs to COG1826 (Galperin et al., 2021)

Gene

Coordinates: 285,775 → 285,987

The protein

Catalyzed reaction/ biological activity

• translocation of PhoD PubMed

Protein family

• TatA/E family (with TatAC and TatAY, according to UniProt)

Structure

• 2L16 (PDB) PubMed
• O31467 (AlphaFold)

Paralogous protein(s)

• TatAY, TatAC

Localization

• membrane (according to Swiss-Prot),  forms foci at the division sites and cell poles, localization requires interaction with TatCD or TatCY PubMed

Expression and Regulation

Operons

• Genes: phoD-tatAD-tatCD

  Description: PubMed

  Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PubMed, in phoP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • expression of the operon depends on functional HemAT and CsbC PubMed

Biological materials

Mutant

• BKE02630 (ΔtatAD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTTGAAAACATAATTTCCA,  downstream forward: _UP4_TGAATGCTGATGAGGCAGAC
• BKK02630 (ΔtatAD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTTTGAAAACATAATTTCCA,  downstream forward: _UP4_TGAATGCTGATGAGGCAGAC

Labs working on this gene/protein

• Jan Maarten van Dijl, University of Groningen, The Netherlands, Homepage

References

Reviews

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Original Publications

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