hrcA

168

transcriptional repressor of class I heat-shock genes

Locus: BSU_25490

Molecular weight: 38.87 kDa

Isoelectric point: 6.08

Protein length: 343 aa

Gene length: 1032 bp

Function: regulation of chaperone gene expression

Product: transcriptional repressor

Essential: no

Synonyms: hrcA, yqxE, yqeS

Genomic Context

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List of homologs in different organisms, belongs to COG1420 (Galperin et al., 2021)

Gene

Coordinates: 2,628,606  2,629,637

Phenotypes of a mutant

• reduced survival after DNA replication arrest imposed by inhibition of PolC activity PubMed

The protein

Catalyzed reaction/ biological activity

• transcription repressor of the ''hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV and groES-groEL'' operons (at low/ normal temperatures)

Protein family

• hrcA family (single member, according to UniProt)

Cofactors

• GroEL acts as co-repressor

Structure

• 1STZ (PDB) (from Thermotoga maritima, 27% identity) PubMed
• P25499 (AlphaFold)

Modification

• phosphorylated on arginine residues PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hrcA-grpE-dnaK-dnaJ-yqeT-yqeU-yqeV

  Description: PubMed

  Regulation

  • induced by heat shock (HrcA) PubMed

  Regulatory mechanism

  • HrcA: repression, PubMed, in hrcA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE25490 (hrcA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATCATCACCTCTGTTA,  downstream forward: _UP4_TAAGGGAATTTTGGCAAATT
• BKK25490 (hrcA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATCATCACCTCTGTTA,  downstream forward: _UP4_TAAGGGAATTTTGGCAAATT

Labs working on this gene/protein

• Wolfgang Schumann, Bayreuth University, Germany Homepage
• Thomas Wiegert, University of Bayreuth, Germany Wiegert-Dateien/Thomas Wiegert.html Homepage

References

Reviews

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Original Publications

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