topB

168

DNA topoisomerase III, decatenase

Locus: BSU_04260

Molecular weight: 81.29 kDa

Isoelectric point: 9.88

Protein length: 727 aa

Gene length: 2184 bp

Function: control of DNA recombination, maintenance of genomic stability

Product: DNA topoisomerase III

Essential: no

Synonyms: topB

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Information processing, Genetics, DNA replication

Information processing, Genetics, DNA condensation/ segregation

List of homologs in different organisms, belongs to COG0551 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene

Coordinates: 476,558  478,741

Phenotypes of a mutant

in E. coli, deletion results in a mutator phenotype PubMed

The protein

Catalyzed reaction/ biological activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining (according to UniProt)
binds to abasic and RNA base DNA lesions PubMed

Protein family

Type IA topoisomerase family (together with TopB)(according to UniProt)

Domains

N-terminal TOPRIM domain (aa 3-136) PubMed

Structure

1D6M (PDB) (from E. coli, 33% identity) PubMed
P96583 (AlphaFold)

Effectors of protein activity

RecQ controls Topo III (de)catenation activity PubMed

Paralogous protein(s)

TopA

Expression and Regulation

Operons

Genes: topB

Description

PubMed

Genes: lrpC-topB

Description

PubMed

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

MGNA-C111 (topB::erm), available at the NBRP B. subtilis, Japan
GP1864 (ΔtopB::ermC) (available in Jörg Stülke's lab)
GP1896 (ΔtopB::cat) (available in Jörg Stülke's lab)
JH642 and its derivatives: natural deletion of the 18 kb ydzA-mntH region encompassing the genes ydzA-topB-ydaJ-ydaK-ydaL-ydaM-ydaN-kimA-mutT-ydaP-ydzK-mntH PubMed
BKE04260 (ΔtopB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTGATCACTTCTCTT,  downstream forward: _UP4_TAATAAAAAAACCTGTGCCT
BKK04260 (ΔtopB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTTGATCACTTCTCTT,  downstream forward: _UP4_TAATAAAAAAACCTGTGCCT

References

Reviews

Loading

Original Publications

Loading