glpK

168

glycerol kinase

Locus: BSU_09290

Molecular weight: 54.91 kDa

Isoelectric point: 4.99

Protein length: 496 aa

Gene length: 1491 bp

Function: glycerol utilization

Product: glycerol kinase

Essential: no

E.C.: 2.7.1.30

Synonyms: glpK

Genomic Context

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List of homologs in different organisms, belongs to COG0554 (Galperin et al., 2021)

Gene

Coordinates: 1,003,344 → 1,004,834

The protein

Catalyzed reaction/ biological activity

• ATP + glycerol --> ADP + H⁺ + sn-glycerol 3-phosphate (according to UniProt)

Protein family

• FGGY kinase family (according to UniProt)

Structure

• 3H46 (PDB) (from Enterococcus casseliflavus, complex with glycerol) PubMed
• P18157 (AlphaFold)

Modification

• phosphorylation (His230) (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: glpF-glpK

  Description: PubMed

  Regulation

  • induction by glycerol (GlpP) PubMed
  • repressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • GlpP: antitermination, /antitermination via GlpP-dependent RNA switch, in glpP regulon
  • AbrB: activation, PubMed, in abrB regulon
  • ResD: repression, PubMed, in resD regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE09290 (glpK::ermC) (available at the BGSC and in Jörg Stülke's lab) PubMed
• GP1865 (glpK::ermC) (available in Jörg Stülke's lab)
• BKE09290 (ΔglpK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCATAAGATGTCTCCCCT,  downstream forward: _UP4_TAAAGTAATACTATGGTATA
• BKK09290 (ΔglpK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCCATAAGATGTCTCCCCT,  downstream forward: _UP4_TAAAGTAATACTATGGTATA

Labs working on this gene/protein

• Josef Deutscher, Paris-Grignon, France

References

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