ilvA

168

threonine dehydratase

Locus: BSU_21770

Molecular weight: 46.49 kDa

Isoelectric point: 5.54

Protein length: 422 aa

Gene length: 1266 bp

Function: biosynthesis of branched-chain amino acids

Product: threonine dehydratase

Essential: no

E.C.: 4.3.1.19

Synonyms: ilvA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1171 (Galperin et al., 2021)

Gene

Coordinates: 2,292,769 → 2,294,037

The protein

Catalyzed reaction/ biological activity

• L-threonine --> 2-oxobutanoate + NH₄⁺ (according to UniProt)

Protein family

• serine/threonine dehydratase family (with DsdA, according to UniProt)

Domains

• ACT-like domain (aa 339-413) (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 1TDJ (PDB) (the enzyme from E. coli, 39% identity) PubMed
• P37946 (AlphaFold)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: ilvA-ypmP

  Description: PubMed

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

Biological materials

Mutant

• BKE21770 (ΔilvA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTACAGATTCCTTTCTT,  downstream forward: _UP4_TAATTTTTTTACACAACCGC
• BKK21770 (ΔilvA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTACAGATTCCTTTCTT,  downstream forward: _UP4_TAATTTTTTTACACAACCGC

LacZ fusion

• pGP519 (in pAC5), available in Stülke lab

References

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