ilvH

168

acetolactate synthase (small subunit)

Locus: BSU_28300

Molecular weight: 19.31 kDa

Isoelectric point: 10.3

Protein length: 172 aa

Gene length: 519 bp

Function: biosynthesis of branched-chain amino acids

Product: acetolactate synthase (small subunit)

Essential: no

E.C.: 2.2.1.6

Synonyms: ilvH, ilvN

Genomic Context

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Categories containing this gene/protein

Metabolism, Amino acid/ nitrogen metabolism, Biosynthesis/ acquisition of amino acids, Biosynthesis/ acquisition of branched-chain amino acids

List of homologs in different organisms, belongs to COG0440 (Galperin et al., 2021)

Gene

Coordinates: 2,894,733  2,895,251

The protein

Catalyzed reaction/ biological activity

H⁺ + 2 pyruvate --> (2S)-2-acetolactate + CO₂ (according to UniProt)

Protein family

acetolactate synthase small subunit family (single member, according to UniProt)

Domains

ACT domain (aa 4-79) (according to UniProt)

Structure

2F1F (PDB) (regulatory subunit from Escherichia coli, 37% identity, 62% similarity) PubMed
P37252 (AlphaFold)

Effectors of protein activity

subject to feedback inhibition by valine PubMed

Expression and Regulation

Operons

Genes: ilvB-ilvH-ilvC-leuA-leuB-leuC-leuD

Description: PubMed

Regulation

expression is stimulated in the presence of glucose (CcpA) PubMed

Regulatory mechanism

T-box: RNA switch, via tRNA controlled RNA switch, repression by BCAA, in T-box
TnrA: repression, PubMed, in tnrA regulon
CodY: repression, PubMed PubMed, in codY regulon
CcpA: activation, PubMed, in ccpA regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Additional information

An antisense RNA is predicted for leuA PubMed
the T-box RNA is degraded by RNase Y PubMed

Biological materials

Mutant

BKE28300 (ilvH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTCAATGTGATAATTC,  downstream forward: _UP4_TAAAACATAACAAGGGAGAG
BKK28300 (ilvH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTCAATGTGATAATTC,  downstream forward: _UP4_TAAAACATAACAAGGGAGAG

References

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