degU

degU
168

two-component response regulator, regulation of degradative enzyme expression, genetic competence, biofilm formation, capsule biosynthesis (together with SwrA), non-phosphorylated DegU is required for swarming motility

Locus
BSU_35490
Molecular weight
25.72 kDa
Isoelectric point
5.6
Protein length
Gene length
Function
regulation of degradative enzymes, genetic competence, and other adaptive responses
Product
two-component response regulator
Essential
no
Synonyms
degU, sacU, iep

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG2197 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
3,644,607 → 3,645,296
Phenotypes of a mutant
the degU gene is readily inactivated during domestication of B. subtilis PubMed
loss of genetic competence, competence can be restored by overexpression of comK-comS PubMed
defect in biofilm formation PubMed, this can be suppressed by DegU-independent expression of BslA PubMed
the mutation suppresses the mucoid phenotype of motA or motB mutants due to reduced expression of the capB-capC-capA-capE operon PubMed
defective in Swarming motility PubMed
The protein
Catalyzed reaction/ biological activity
transcription activator and repressor (see DegU regulon)
DegU-P represses expression of the fla/che operon (''flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB'') in the absence of SwrAA/2, and switches to an activator of the operon in the presence of SwrAA/2 PubMed
Response regulatory domain (aa 5-121) (according to UniProt)
HTH luxR-type domain (aa 159-224) (according to UniProt)
Structure
4GVP (PDB) (VraR from Staphylococcus aureus, 35% identity) PubMed
Modification
phosphorylated by DegS on Asp-56 PubMed, this modulates DNA-binding activity
phosphorylation by DegS occurs in response to inhibition of flagellar rotation PubMed
Effectors of protein activity
DNA-binding activity of DegU is inhibited by RapG PubMed
transcription activation of the fla/che promoter by DegU-P is facilitated by interaction with SwrA PubMed
DegU-P is degraded by ClpC-ClpP PubMed
cytoplasm (according to Swiss-Prot)
Expression and Regulation
Operons
Genes
Description
Regulation
induced by glucose (CcpA) PubMed
Regulatory mechanism
DegU: activation, in degU regulon
TnrA: repression, PubMed, in tnrA regulon
CcpA: activation, PubMed, in ccpA regulon
SinR: repression, PubMed, in sinR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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degSdegU

2024-12-04 21:28:41

Bzhu

111

d2120c3a90e78aa86a7316960e6c6ac9791f1ad9

180E2E6A277BD691F2D486B91512361FC857D302

Genes
Description
Regulation
induced by glucose (CcpA) PubMed
Open in new tab

degU

2024-11-19 23:45:32

Bzhu

105

257f1a2d62e3fc92fcf9c13c9a66eca2363796ce

B7D54FCF42D7EC3DDEBA1671E67F93E16CF6A20E

Biological materials
Mutant
GP2644 (ΔdegU::aphA3) available in Jörg Stülke's lab PubMed
BKE35490 (ΔdegU::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT,  downstream forward: _UP4_TAGTATAATAGGAGACTTGC
BKK35490 (ΔdegU::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACAAGCCACGCCTCCTTGT,  downstream forward: _UP4_TAGTATAATAGGAGACTTGC
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
References
Reviews
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Original Publications
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D343D2096664A972026D911E7A17A35C7B1CD1C9

Page visits: 14891

Time of last update: 2024-12-23 13:31:51

Author of last update: Jstuelk