appD

168

oligopeptide ABC transporter (ATP-binding protein)

Locus: BSU_11360

Molecular weight: 36.16 kDa

Isoelectric point: 5.39

Protein length: 328 aa

Gene length: 987 bp

Function: uptake of oligopeptides

Product: oligopeptide ABC transporter (ATP-binding protein))

Essential: no

Synonyms: appD

Genomic Context

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List of homologs in different organisms, belongs to COG0444 (Galperin et al., 2021)

Gene

Coordinates: 1,211,477  1,212,463

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 5-256) (according to UniProt)

Structure

• 4FWI (PDB) (from Caldanaerobacter subterraneus, 38% identity) PubMed
• P42064 (AlphaFold)

Paralogous protein(s)

• DppD, YkfD, OppD

Localization

• attached to the cell membrane (via AppB-AppC) PubMed

Expression and Regulation

Operons

• Genes: appD-appF-appA/1-appA/2-appB-appC

  Description: PubMed

  Regulation

  • repressed by CodY PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon
  • CodY: repression, PubMed PubMed, in codY regulon
  • ScoC: repression, PubMed, in scoC regulon

Biological materials

Mutant

• GP2100 (D(''appD-appF-appA/2-appB-appC)::spc''), available in Jörg Stülke's lab
• BKE11360 (appD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTAGCTCCCCTTTCCC,  downstream forward: _UP4_GAGGAAGAGGGTGCCGAACA
• BKK11360 (appD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCTAGCTCCCCTTTCCC,  downstream forward: _UP4_GAGGAAGAGGGTGCCGAACA

References

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