pucK

168

uric acid permease

Locus: BSU_32440

Molecular weight: 44.70 kDa

Isoelectric point: 8.35

Protein length: 430 aa

Gene length: 1293 bp

Function: purine utilization

Product: uric acid permease

Essential: no

Synonyms: pucK, yunK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2233 (Galperin et al., 2021)

Gene

Coordinates: 3,331,857  3,333,149

The protein

Protein family

• xanthine/uracil permease family (according to UniProt)
• Nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) subfamily (according to UniProt)

Structure

• 3QE7 (PDB) (E. coli uracil transporter, 28% identity) PubMed
• O32140 (AlphaFold)

Paralogous protein(s)

• PucJ, PbuX

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: pucJ-pucK-pucL-pucM

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon
  • PucR: activation, PubMed, in pucR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: pucR-pucJ-pucK-pucL-pucM

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • PucR: auto-repression, PubMed, in pucR regulon
  • TnrA: activation, PubMed, in tnrA regulon

Biological materials

Mutant

• MGNA-A936 (yunK::erm), available at the NBRP B. subtilis, Japan
• BKE32440 (pucK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTGTGCTGTTCCTTCATCT,  downstream forward: _UP4_ATAAAAACGTAAAGGAAGAT
• BKK32440 (pucK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTGTGCTGTTCCTTCATCT,  downstream forward: _UP4_ATAAAAACGTAAAGGAAGAT
• BP1257(pucJK::cat trp+) available in Jörg Stülke's & Fabian Commichau's lab

References

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