oppF

168

oligopeptide ABC transporter (ATP-binding protein)

Locus: BSU_11470

Molecular weight: 34.59 kDa

Isoelectric point: 6.95

Protein length: 305 aa

Gene length: 918 bp

Function: initiation of sporulation, competence development

Product: oligopeptide ABC transporter (ATP-binding protein)

Essential: no

Synonyms: oppF, spo0KE

Genomic Context

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List of homologs in different organisms, belongs to COG1123 (Galperin et al., 2021)

Gene

Coordinates: 1,224,533  1,225,450

The protein

Catalyzed reaction/ biological activity

• required for the uptake of quorum sensing peptides such as PhrH PubMed

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 6-251) (according to UniProt)

Structure

• 3DHW (PDB) (MetN from E. coli, 33% identity) PubMed
• P24137 (AlphaFold)

Paralogous protein(s)

• AppF, YkfD

Localization

• attached to the cell membrane (via OppB-OppC) PubMed

Expression and Regulation

Operons

• Genes: oppA-oppB-oppC-oppD-oppF

  Description: PubMed

  Regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon
  • CodY: repression, no derepression occurs, however, in a CodY mutant, due to increased repression by ScoC PubMed, in codY regulon
  • ScoC: repression, PubMed, in scoC regulon

  Additional information

  • A ncRNA is predicted between 'trpS' and 'oppA' PubMed

Biological materials

Mutant

• GP2097 (D(''oppA-oppB-oppC-oppD-oppF)::aphA3''), available in Jörg Stülke's lab
• BKE11470 (oppF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTCATTCAACTTCTCTC,  downstream forward: _UP4_TGATTCATCAATCCTTCAAG
• BKK11470 (oppF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTCATTCAACTTCTCTC,  downstream forward: _UP4_TGATTCATCAATCCTTCAAG

References

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