nrgB

168

nitrogen-regulated PII protein

Locus: BSU_36520

Molecular weight: 12.68 kDa

Isoelectric point: 8.82

Protein length: 116 aa

Gene length: 351 bp

Function: regulation of ammonium uptake

Product: nitrogen-regulated PII protein

Essential: no

Synonyms: nrgB, glnK

Genomic Context

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List of homologs in different organisms, belongs to COG0347 (Galperin et al., 2021)

Gene

Coordinates: 3,758,016 → 3,758,366

The protein

Protein family

• P(II) protein family (single member, according to UniProt)

Structure

• 4R25 (PDB) PubMed
• 4R25 PubMed
• 2NUU (the complex with NrgA, from E. coli) PubMed
• Q07428 (AlphaFold)

Localization

• cell membrane, via NrgA PubMed

Expression and Regulation

Operons

• Genes: nrgA-nrgB

  Description: PubMed

  Regulation

  • induced by ammonium limitation

  Regulatory mechanism

  • TnrA: activation, PubMed, in tnrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• GP4167 (ΔnrgA-nrgB::spec), available in  Jörg Stülke's lab
• GP4168 (ΔnrgB::phleo), available in  Jörg Stülke's lab
• GP259 (ΔnrgB::cat), available in Jörg Stülke's lab PubMed
• GP255 (ΔnrgA-nrgB::cat), available in Jörg Stülke's lab PubMed
• GP4092 (ΔnrgB::phleo), available in Jörg Stülke's lab
• BKE36520 (ΔnrgB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC,  downstream forward: _UP4_TAATATCGGTACGAGATTCG
• BKK36520 (ΔnrgB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTGACCGCTCATAGCGTCAC,  downstream forward: _UP4_TAATATCGGTACGAGATTCG

Antibody

• available in Jörg Stülkes lab PubMed

Labs working on this gene/protein

• Susan Fisher, Boston, USA homepage

References

Reviews

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Original Publications

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