nasF

168

uroporphyrinogen methyltransferase

Locus: BSU_03280

Molecular weight: 53.70 kDa

Isoelectric point: 6.52

Protein length: 483 aa

Gene length: 1452 bp

Function: nitrate respiration

Product: uroporphyrinogen methyltransferase

Essential: no

E.C.: 2.1.1.107

Synonyms: nasF, nasBE

Genomic Context

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List of homologs in different organisms, belongs to COG1587 (Galperin et al., 2021)

Gene

Coordinates: 353,900  355,351

The protein

Catalyzed reaction/ biological activity

• 2 S-adenosyl-L-methionine + uroporphyrinogen III --> H⁺ + precorrin-2 + 2 S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• precorrin methyltransferase family (with YlnD, according to UniProt)

Structure

• 1PJQ (PDB) (CysG from Salmonella enterica, 46% identity to N-terminal domain) PubMed
• P42437 (AlphaFold)

Paralogous protein(s)

• YlnD, (corresponds to N-terminal domain of NasF)

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF

  Description: PubMed

  Regulation

  • ''nasD'': expressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in resD regulon
  • TnrA: activation, PubMed, in tnrA regulon
  • NsrR: repression, PubMed, in nsrR regulon
  • Fur: repression, PubMed, in fur regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: nasD-nasE-nasF

  Description: PubMed

  Regulation

  • ''nasD'': expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• BKE03280 (nasF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATCCTTCCGTCACT,  downstream forward: _UP4_TGATGGCAAGCAGCCCTTTT
• BKK03280 (nasF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTCATCCTTCCGTCACT,  downstream forward: _UP4_TGATGGCAAGCAGCCCTTTT

References

Reviews

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Original Publications

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