nasD
168
assimilatory nitrite reductase (subunit)
Locus
BSU_03300
Molecular weight
88.24 kDa
Isoelectric point
4.95
Function
utilization of nitrite as nitrogen source
Product
assimilatory nitrite reductase (subunit)
Essential
no
E.C.
1.7.1.4
Synonyms
nasD, nasBC, nirB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
355,764 → 358,181
The protein
Catalyzed reaction/ biological activity
2 H2O + 3 NADP+ + NH4+ --> 5 H+ + 3 NADPH + nitrite (according to UniProt)
2 H2O + 3 NAD+ + NH4+ --> 5 H+ + 3 NADH + nitrite (according to UniProt)
Protein family
nitrite and sulfite reductase 4Fe-4S domain family (with CysI, according to UniProt)
Structure
3KLJ (PDB) (from Clostridium acetobutylicum, corresponds to aa 5 ... 399, 32% identity) PubMed
Paralogous protein(s)
Expression and Regulation
Operons
Biological materials
Mutant
References
Reviews
Bacterial nitrate assimilation: gene distribution and regulation.
Biochemical Society transactions. 2011 Dec; 39(6):1838-43. doi:10.1042/BST20110688. PMID:22103536
Functional, biochemical and genetic diversity of prokaryotic nitrate reductases.
Cellular and molecular life sciences : CMLS. 2001 Feb; 58(2):165-78. . PMID:11289299
Original Publications
Protein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Distinct Interaction Mechanism of RNAP and ResD and Distal Subsites for Transcription Activation of Nitrite Reductase in Bacillus subtilisψ.Journal of bacteriology. 2021 Dec 13; :JB0043221. PMID: 34898263
Genome-wide Identification of ResD, NsrR, and Fur Binding in Bacillus subtilis during Anaerobic Fermentative Growth by in vivo Footprinting.
Journal of bacteriology. 2017 Apr 24; . pii:JB.00086-17. doi:10.1128/JB.00086-17. PMID:28439033
Genome-wide mapping of TnrA-binding sites provides new insights into the TnrA regulon in Bacillus subtilis.
MicrobiologyOpen. 2015 Jun; 4(3):423-35. doi:10.1002/mbo3.249. PMID:25755103
The ResD response regulator, through functional interaction with NsrR and fur, plays three distinct roles in Bacillus subtilis transcriptional control.
Journal of bacteriology. 2014 Jan; 196(2):493-503. doi:10.1128/JB.01166-13. PMID:24214949
Nitric oxide-sensitive and -insensitive interaction of Bacillus subtilis NsrR with a ResDE-controlled promoter.
Molecular microbiology. 2010 Dec; 78(5):1280-93. doi:10.1111/j.1365-2958.2010.07407.x. PMID:21091510
Crystal structure of NADH:rubredoxin oxidoreductase from Clostridium acetobutylicum: a key component of the dioxygen scavenging system in obligatory anaerobes.
Proteins. 2010 Mar; 78(4):1066-70. doi:10.1002/prot.22650. PMID:20017214
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
Journal of bacteriology. 2006 Aug; 188(16):5878-87. . PMID:16885456
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Molecular microbiology. 2003 Jul; 49(1):157-65. . PMID:12823818
Interaction of ResD with regulatory regions of anaerobically induced genes in Bacillus subtilis.
Molecular microbiology. 2000 Sep; 37(5):1198-207. . PMID:10972836
Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE.
Journal of bacteriology. 1998 Oct; 180(20):5344-50. . PMID:9765565
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proceedings of the National Academy of Sciences of the United States of America. 1996 Aug 20; 93(17):8841-5. . PMID:8799114
The nasB operon and nasA gene are required for nitrate/nitrite assimilation in Bacillus subtilis.
Journal of bacteriology. 1995 Mar; 177(5):1409-13. . PMID:7868621
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Time of last update: 2025-06-11 16:41:07
Author of last update: Jstuelk