nasD

168

assimilatory nitrite reductase (subunit)

Locus: BSU_03300

Molecular weight: 88.24 kDa

Isoelectric point: 4.95

Protein length: 805 aa

Gene length: 2418 bp

Function: utilization of nitrite as nitrogen source

Product: assimilatory nitrite reductase (subunit)

Essential: no

E.C.: 1.7.1.4

Synonyms: nasD, nasBC, nirB

Genomic Context

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List of homologs in different organisms, belongs to COG1251 (Galperin et al., 2021)

Gene

Coordinates: 355,764 → 358,181

The protein

Catalyzed reaction/ biological activity

• 2 H₂O + 3 NADP⁺ + NH₄⁺ --> 5 H⁺ + 3 NADPH + nitrite (according to UniProt)
• 2 H₂O + 3 NAD⁺ + NH₄⁺ --> 5 H⁺ + 3 NADH + nitrite (according to UniProt)

Protein family

• nitrite and sulfite reductase 4Fe-4S domain family (with CysI, according to UniProt)

Cofactors

• Fe-S cluster  PubMed
• FAD  PubMed

Structure

• 3KLJ (PDB) (from Clostridium acetobutylicum, corresponds to aa 5 ... 399, 32% identity) PubMed
• P42435 (AlphaFold)

Paralogous protein(s)

• NasB

Expression and Regulation

Operons

• Genes: nasB-nasC-nasD-nasE-nasF

  Description: PubMed

  Regulation

  • ''nasD'': expressed under anaerobic conditions (ResD) PubMed

  Regulatory mechanism

  • ResD: activation, PubMed, in resD regulon
  • TnrA: activation, PubMed, in tnrA regulon
  • NsrR: repression, PubMed, in nsrR regulon
  • Fur: repression, PubMed, in fur regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: nasD-nasE-nasF

  Description: PubMed

  Regulation

  • ''nasD'': expressed under anaerobic conditions (ResD) PubMed

Biological materials

Mutant

• 1A973 ( nasD::phleo), PubMed, available at BGSC
• BKE03300 (ΔnasD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGATGATCCGCTCCTT,  downstream forward: _UP4_TAATGACAAAAACTATCATT
• BKK03300 (ΔnasD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAGATGATCCGCTCCTT,  downstream forward: _UP4_TAATGACAAAAACTATCATT

References

Reviews

Luque-Almagro VM, Gates AJ, Moreno-Vivián C, Ferguson SJ, Richardson DJ, Roldán MD Bacterial nitrate assimilation: gene distribution and regulation. Biochemical Society transactions. 2011 Dec; 39(6):1838-43. doi:10.1042/BST20110688. PMID:22103536
Richardson DJ, Berks BC, Russell DA, Spiro S, Taylor CJ Functional, biochemical and genetic diversity of prokaryotic nitrate reductases. Cellular and molecular life sciences : CMLS. 2001 Feb; 58(2):165-78. . PMID:11289299

Original Publications

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