nusA

168

transcription termination factor of RNA polymerase, facilitates the formation of weak terminator hairpins and compensates for distal U-rich tract interruptions

Locus: BSU_16600

Molecular weight: 41.57 kDa

Isoelectric point: 4.58

Protein length: 371 aa

Gene length: 1116 bp

Function: transcription

Product: transcription termination factor

Essential: yes

Synonyms: nusA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0195 (Galperin et al., 2021)

Gene

Coordinates: 1,732,281  1,733,396

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• stimulates transcription termination at weak intrinsic terminators PubMed

Protein family

• NusA family (single member, according to UniProt)

Domains

• S1 domain (aa 133-199) (according to UniProt)
• KH domain (aa 301-367) (according to UniProt)

Structure

• 1HH2 (PDB) (from Thermotoga maritima, 51% identity) PubMed
• P32727 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: ylxS-nusA-rnpM-rplGA-infB-ylxP-rbfA

  Description: PubMed

  Regulation

  • autoregulation NusA PubMed
  • induced by glucose PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • NusA: attenuation, NusA stimulates termination PubMed, in nusA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's and Jörg Stülke's lab

References

Reviews

Loading

Original Publications

Loading