walR

168

two-component response regulator (OmpR family), controls cell wall metabolism

Locus: BSU_40410

Molecular weight: 27.08 kDa

Isoelectric point: 4.88

Protein length: 235 aa

Gene length: 708 bp

Function: control of cell wall metabolism

Product: two-component response regulator (OmpR family)

Essential: yes

Synonyms: walR, yycF

Genomic Context

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List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 4,153,696  4,154,403

Phenotypes of a mutant

• essential PubMed
• essentiality can be bypassed by constitutive expression of lytE or cwlO in a pdaC iseA mutant background PubMed
• a constitutively active form of WalR (WalR-R204C) results in constitutive expression of the WalR regulon, and in combination with a ''sepF'' mutation to the formation of cell wall-less L-forms  PubMed

The protein

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 4-117) (according to UniProt)

Structure

• 2OQR (PDB) (from Mycobacterium tuberculosis, 48% identity) PubMed
• 2D1V (PDB) (DNA binding domain)2ZWM (PDB) (receiver domain)
• P37478 (AlphaFold)

Modification

• phosphorylated by WalK on Asp-53
• phosphorylated by PrkC on Thr-101, this stimulates WalR activity PubMed

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Paralogous protein(s)

• YkoG, YclJ, PhoP

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: walR-walK-walH-walI-walJ-htrC

  Description: PubMed

  Regulation

  • htrC transcript expressed during sporulation PubMed

Biological materials

Mutant

• MGNA-B828 (yycF::erm), available at the NBRP B. subtilis, Japan

Expression vectors

• pGP2633, constitutive expression in B. subtilis (based on pBQ200), available in Jörg Stülke's lab
• pGP2635, expression of His-WalR in E. coli (based on pWH844), available in Jörg Stülke's lab

Labs working on this gene/protein

• Kevin Devine, Dublin

References

Reviews

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Original Publications

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The WalR regulon

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