prkC

168

protein kinase C, fine-tuning of cell growth rates in response to Lipid II in different nutrient conditions, induces Germination of spores in response to DAP-type, and not to Lys-type cell wall muropeptides, stimulates WalR activity

Locus: BSU_15770

Molecular weight: 71.69 kDa

Isoelectric point: 4.83

Protein length: 648 aa

Gene length: 1947 bp

Function: regulation of growth and germination in response to muropeptides and Lipid II

Product: protein kinase

Essential: no

E.C.: 2.7.11.1

Synonyms: prkC, yloP

Genomic Context

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List of homologs in different organisms, belongs to COG2815 (Galperin et al., 2021)

Gene

Coordinates: 1,651,142  1,653,088

Phenotypes of a mutant

• unable to germinate in response to muropeptides PubMed
• reduced growth at high salt PubMed

The protein

Catalyzed reaction/ biological activity

• phosphorylation of WalR on Thr-101 to stimulate its activity PubMed
• phosphorylates PrkA on Thr-217 PubMed
• phosphorylation of RodZ in response to lipid II abundance PubMed

Protein family

• protein kinase superfamily (according to UniProt)
• Ser/Thr protein kinase family (according to UniProt)

Domains

• contains three C-terminal PASTA domains (aa 356-424, 425-492, 493-559) (binds muropeptides) PubMed
• Protein kinase domain (aa 11-271) (according to UniProt)

Structure

• 4EQM (PDB) (intracellular domain of the Staphylococcus aureus enzyme, 51% identity) PubMed
• 3PY9 (PDB) (entire extra-cellular region of PrkC from Staphylococcus aureus)  PubMed
• 4X3F (PDB) (intracellular domain of the Mycobacterium tuberculosis enzyme, 36% identity, 68% similarity) PubMed
• O34507 (AlphaFold)

Modification

• phosphorylation on Thr-290 PubMed, autophosphorylation on multiple threonine residues PubMed

Effectors of protein activity

• activated by muropeptides PubMed
• lipid II PubMed
• GpsB, DivIVA, and EzrA are required for stimulation of PrkC activity PubMed

Localization

• inner spore membrane PubMed
• membrane PubMed
• division septum PubMed

Expression and Regulation

Operons

• Genes: prpC-prkC-cpgA-rpeA-yloS

  Description: PubMed

Biological materials

Mutant

• MGNA-B134 (yloP::erm), available at the NBRP B. subtilis, Japan
• GP576 (spc), OMG302 (aphA3), available in Jörg Stülke's lab
• 1A820 ( prkC::erm), PubMed, available at BGSC
• 1A963 (no resistance), PubMed, available at BGSC
• 1A964 (no resistance), PubMed, available at BGSC
• BKE15770 (prkC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATTAGCACTGATCTTCACC,  downstream forward: _UP4_GATGAATAACAAGGAGGGAA
• BKK15770 (prkC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GATTAGCACTGATCTTCACC,  downstream forward: _UP4_GATGAATAACAAGGAGGGAA

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP832, available in Jörg Stülke's lab
• for expression/ purification of the kinase domain from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP849, available in Jörg Stülke's lab
• for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1001,  available in Jörg Stülke's lab
• for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP825,  available in Jörg Stülke's lab, PubMed

LacZ fusion

• pGP829 (in pAC7), available in Jörg Stülke's lab

References

Reviews

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Phosphorylation of PrkC

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Phsiological role of PrkC

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Structure/ biochemistry of PrkC

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Expression/ localization of PrkC

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Targets of PrkC-dependent phosphorylation

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