ytvA

168

blue light sensor, positive regulation of SigB activity under conditions of blue light

Locus: BSU_30340

Molecular weight: 29.04 kDa

Isoelectric point: 4.69

Protein length: 261 aa

Gene length: 786 bp

Function: control of SigB activity

Product: blue light receptor, flavoprotein

Essential: no

Synonyms: ytvA

Genomic Context

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List of homologs in different organisms, belongs to COG2202 (Galperin et al., 2021)

Gene

Coordinates: 3,106,210  3,106,995

The protein

Catalyzed reaction/ biological activity

• mediates light-dependent stimulation of RsbT kinase activity PubMed

Domains

• PAS domain (aa 12-87) (according to UniProt)
• PAC domain (aa 88-138) (according to UniProt)
• STAS domain (aa 147-258) (according to UniProt)

Cofactors

• FMN

Structure

• 2PR5 (PDB) (dark),  2PR6 (PDB) (light), 2MWG (PDB)
• O34627 (AlphaFold)

Effectors of protein activity

• YtvA-dependent light activation of the SigB stress response is positively and negatively regulated by RsbR and RsbRB, respectively PubMed

Paralogous protein(s)

• YtvA, RsbRD, RsbRB, RsbRC, RsbR

Expression and Regulation

Operons

• Genes: ytvA

  Description: PubMed

  Expression

  • ytvA expression shows a circadian behaviour PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

Biological materials

Mutant

• MGNA-A136 (ytvA::erm), available at the NBRP B. subtilis, Japan
• 1A965 ( ytvA::erm), PubMed, available at BGSC
• BKE30340 (ytvA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAATCCCCCTTAGGC,  downstream forward: _UP4_TAAAAAGATCCCGCTCACCC
• BKK30340 (ytvA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAATCCCCCTTAGGC,  downstream forward: _UP4_TAAAAAGATCCCGCTCACCC

Expression vectors

• pGP2685, expression of His-YtvA in E. coli (based on pGP570), available in Jörg Stülke's lab

Labs working on this gene/protein

• Chet Price, Davis, USA homepage

References

Reviews

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Original Publications

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