dltC

168

D-alanine carrier protein, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides

Locus: BSU_38520

Molecular weight: 8.87 kDa

Isoelectric point: 3.62

Protein length: 78 aa

Gene length: 237 bp

Function: biosynthesis of teichoic acid

Product: D-alanine carrier protein

Essential: no

E.C.: 6.1.1.13

Synonyms: dltC, ipa-3r

Genomic Context

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List of homologs in different organisms, belongs to COG0236 (Galperin et al., 2021)

Gene

Coordinates: 3,954,987  3,955,223

Phenotypes of a mutant

• more sensitive to nisin PubMed

The protein

Catalyzed reaction/ biological activity

• receives D-alanine from DltA PubMed

Protein family

• DltC family (single member, according to UniProt)

Domains

• Carrier domain (aa 1-78) (according to UniProt)

Structure

• 4BPG (PDB) PubMed
• P39579 (AlphaFold)

Modification

• modified with the 4-phosphopantetheine (Ppant) group at Ser35, by AcpS PubMed, then further modified with a d-alanyl group by DltA, through a thioester bond PubMed

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: dltX-dltA-dltB-dltC-dltD-dltE

  Description: PubMed

  Regulation

  • expression is reduced in a SigV mutant PubMed
  • the mRNA is processed between dltX and dltA by RNase Y, this requires the RicA-RicF-RicT complex PubMed

  Regulatory mechanism

  • YvrHb: activation, PubMed, in yvrHb regulon
  • Spo0A: repression, PubMed, in spo0A regulon
  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon
  • SigM: sigma factor, PubMed, in sigM regulon
  • SigX: sigma factor, PubMed, in sigX regulon
  • SigV: sigma factor, PubMed, in sigV regulon

Biological materials

Mutant

• BKE38520 (dltC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT,  downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA
• BKK38520 (dltC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT,  downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA

References

Reviews

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Original Publications

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