dltC

168

D-alanine carrier protein, alanylation of teichoic acid provides some resistance against positively charged antimicrobial peptides

Locus: BSU_38520

Molecular weight: 8.87 kDa

Isoelectric point: 3.62

Protein length: 78 aa

Gene length: 237 bp

Function: biosynthesis of teichoic acid

Product: D-alanine carrier protein

Essential: no

E.C.: 6.1.1.13

Synonyms: dltC, ipa-3r

Genomic Context

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Categories containing this gene/protein

Cellular processes, Cell envelope and cell division, Cell wall synthesis, Biosynthesis of teichoic acid

Metabolism, Additional metabolic pathways, Biosynthesis of cell wall components, Biosynthesis of teichoic acid

Lifestyles, Coping with stress, Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

List of homologs in different organisms, belongs to COG0236 (Galperin et al., 2021)

Gene

Coordinates: 3,954,987  3,955,223

Phenotypes of a mutant

more sensitive to nisin PubMed

The protein

Catalyzed reaction/ biological activity

receives D-alanine from DltA PubMed

Protein family

DltC family (single member, according to UniProt)

Domains

Carrier domain (aa 1-78) (according to UniProt)

Structure

4BPG (PDB) PubMed
P39579 (AlphaFold)

Modification

modified with the 4-phosphopantetheine (Ppant) group at Ser35, by AcpS PubMed, then further modified with a d-alanyl group by DltA, through a thioester bond PubMed

Localization

cytoplasm (according to UniProt)

Expression and Regulation

Operons

Genes: dltX-dltA-dltB-dltC-dltD-dltE

Description: PubMed

Regulation

expression is reduced in a SigV mutant PubMed
the mRNA is processed between dltX and dltA by RNase Y, this requires the RicA-RicF-RicT complex PubMed

Regulatory mechanism

YvrHb: activation, PubMed, in yvrHb regulon
Spo0A: repression, PubMed, in spo0A regulon
stringent response: negative regulation, in stringent response

Sigma factors

SigD: sigma factor, PubMed, in sigD regulon
SigM: sigma factor, PubMed, in sigM regulon
SigX: sigma factor, PubMed, in sigX regulon
SigV: sigma factor, PubMed, in sigV regulon

Biological materials

Mutant

BKE38520 (dltC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT,  downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA
BKK38520 (dltC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAATCCATAGCTTTCTAAT,  downstream forward: _UP4_AATCAGCTGTCTGAGTTGAA

References

Reviews

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Original Publications

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