sinI

168

antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state

Locus: BSU_24600

Molecular weight: 6.47 kDa

Isoelectric point: 6.33

Protein length: 57 aa

Gene length: 174 bp

Function: control of biofilm formation

Product: antagonist of SinR

Essential: no

Synonyms: sinI

Genomic Context

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List of homologs in different organisms

Gene

Phenotypes of a mutant

• altered cell death pattern in colonies PubMed

The protein

Domains

• Sin domain (aa 2-40) (according to UniProt)

Structure

• 5TMX (PDB) PubMed
• 1B0N (PDB) (complex SinR-SinI) PubMed
• P23308 (AlphaFold)

Paralogous protein(s)

• SlrA

Expression and Regulation

Operons

• Genes: sinI-sinR

  Description: PubMed

  Regulation

  • repressed during exponential growth (ScoC) PubMed

  Regulatory mechanism

  • Spo0A: activation, in a portion of cells PubMed, in spo0A regulon
  • SinR: repression, PubMed, in sinR regulon
  • AbrB: repression, PubMed, in abrB regulon
  • ScoC: repression, PubMed, in scoC regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the mRNA is stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• GP959 (spc), available in Jörg Stülke's lab
• DS91 (spc) NCIB3610 derivate, available in Jörg Stülke's lab
• 1S98 ( sinI::kan), PubMed, available at BGSC
• GP1663 (''yqhG-sinI-sinR-tasA''), available in Jörg Stülke's lab
• BKE24600 (sinI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA,  downstream forward: _UP4_TGAATGTGCTATAATATCAC
• BKK24600 (sinI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCAGTTTCTCCTCCTAA,  downstream forward: _UP4_TGAATGTGCTATAATATCAC

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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