cpaA

168

K⁺/H⁺ antiporter

Locus: BSU_11640

Molecular weight: 67.29 kDa

Isoelectric point: 5.25

Protein length: 614 aa

Gene length: 1845 bp

Function: export of potassium ions

Product: K⁺/H⁺ antiporter

Essential: no

Synonyms: cpaA, yjbQ

Genomic Context

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List of homologs in different organisms, belongs to COG0569 (Galperin et al., 2021)

Gene

Coordinates: 1,240,356  1,242,200

The protein

Protein family

• Monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family (with KhtU, according to UniProt)

Domains

• contains a RCK_N domain (aa 403-525) (according to UniProt)
• contains a RCK_C domain at the C-terminus (aa 533-614) (according to UniProt)

Structure

• 4BWZ (PDB) (from Thermus thermophilus, corresponds to the transmembrane domain (aa 27 ... 402), 26% identity) PubMed
• 4YS2 (PDB) (S. aureus CpaA, the RCK_C domain in complex with c-di-AMP, 58% identity)
• O31615 (AlphaFold)

Effectors of protein activity

• binds c-di-AMP, which likely activates export activity PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: cpaA

  Description: PubMed

Biological materials

Mutant

• MGNA-B163 (yjbQ::erm), available at the NBRP B. subtilis, Japan
• GP2082 (''cpaA::ermC''), available in Jörg Stülke's lab
• BKE11640 (cpaA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCAGAACCTCCTTTT,  downstream forward: _UP4_TAAATCTTGACCAAATAAGG
• BKK11640 (cpaA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCAGAACCTCCTTTT,  downstream forward: _UP4_TAAATCTTGACCAAATAAGG

Expression vectors

• pGP2909 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

FLAG-tag construct

• GP2438 yjbQ-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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