spo0F

168

phosphotransferase of the sporulation initiation phosphorelay

Locus: BSU_37130

Molecular weight: 14.09 kDa

Isoelectric point: 4.7

Protein length: 124 aa

Gene length: 375 bp

Function: initiation of sporulation

Product: phosphotransferase of the sporulation initiation phosphorelay

Essential: no

Synonyms: spo0F

Genomic Context

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List of homologs in different organisms, belongs to COG5803 (Galperin et al., 2021)

Gene

Coordinates: 3,809,550  3,809,924

The protein

Domains

• Response regulatory domain (aa 5-119) (according to UniProt)

Structure

• 1SRR (PDB) (phosphatase resistant mutant)
• 1F51 (PDB) (complex with Spo0B)
• 2JVK (PDB) (Mutant L66A),
• 2FSP (PDB)
• 3Q15 (PDB) (RapH-Spo0F complex) PubMed
• P06628 (AlphaFold)

Modification

• phosphorylated on an Asp residue by KinA, KinB, KinC, KinD, or KinE
• dephosphorylated by RapA, RapB, RapE, or RapH PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: spo0F

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigH: sigma factor, PubMed, in sigH regulon

• Genes: spo0F-fbaA-ywjH-murAB-glpX-rho-rpmE

  Description: PubMed

  Expression

  • readthrough-transcription from the SigH-dependent promoter after the initiation of sporulation in the mother cell PubMed

Biological materials

Mutant

• BKE37130 (spo0F::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTACACCCCAATATTA,  downstream forward: _UP4_TGACAAAAAGAAGAAACAAA
• BKK37130 (spo0F::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTACACCCCAATATTA,  downstream forward: _UP4_TGACAAAAAGAAGAAACAAA

References

Reviews

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Original Publications

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