apt

168

adenine phosphoribosyltransferase, universally conserved protein

Locus: BSU_27610

Molecular weight: 18.73 kDa

Isoelectric point: 4.7

Protein length: 170 aa

Gene length: 513 bp

Function: purine salvage and interconversion

Product: adenine phosphoribosyltransferase

Essential: no

E.C.: 2.4.2.7

Synonyms: apt

Genomic Context

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List of homologs in different organisms, belongs to COG0503 (Galperin et al., 2021)

Gene

Coordinates: 2,822,901  2,823,413

The protein

Catalyzed reaction/ biological activity

• AMP + diphosphate --> 5-phospho-α-D-ribose 1-diphosphate + adenine (according to UniProt)

Protein family

• Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)

Structure

• 2DY0 (PDB) (from Escherichia coli k12, 56% identity, 66% similarity)
• O34443 (AlphaFold)

Modification

• phosphorylated on Arg-85 PubMed

Additional information

• universally conserved protein

Expression and Regulation

Operons

• Genes: recJ-apt

  Description: PubMed

  Regulation

  • Rel dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

Biological materials

Mutant

• BKE27610 (apt::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTATTGTTTTAAATCCATCT,  downstream forward: _UP4_TAAGGATAACCGCATAACGA
• BKK27610 (apt::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTATTGTTTTAAATCCATCT,  downstream forward: _UP4_TAAGGATAACCGCATAACGA

References

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