cheB

168

MCP-glutamate methylesterase / two-component response regulator-like

Locus: BSU_16420

Molecular weight: 38.49 kDa

Isoelectric point: 8.12

Protein length: 357 aa

Gene length: 1074 bp

Function: motility and chemotaxis

Product: MCP-glutamate methylesterase / two-component response regulator-like

Essential: no

E.C.: 3.1.1.61

Synonyms: cheB, cheL

Genomic Context

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List of homologs in different organisms, belongs to COG2201 (Galperin et al., 2021)

Gene

Coordinates: 1,711,736 → 1,712,809

Phenotypes of a mutant

• defective in Swarming motility PubMed

The protein

Catalyzed reaction/ biological activity

• [protein]-L-glutamate 5-O-methyl ester + H₂O --> H⁺ + L-glutamyl-[protein] + methanol (according to UniProt)
• H₂O + L-glutaminyl-[protein] --> L-glutamyl-[protein] + NH₄⁺ (according to UniProt)

Protein family

• CheB family (single member, according to UniProt)

Domains

• Response regulatory domain (aa 3-120) (according to UniProt)
• CheB-type methyleserase domain (aa 161-355) (according to UniProt)

Structure

• 1A2O (PDB) (from Salmonella enterica, 41% identity) PubMed
• Q05522 (AlphaFold)

Modification

• phosphorylated on a Asp residue by CheA PubMed

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB

  Description: PubMed

  Regulation

  • see fla-che operon

  Regulatory mechanism

  • Spo0A: repression, in spo0A regulon
  • CodY: repression, in codY regulon
  • DegU: repression, in degU regulon
  • SwrA: activation, in swrA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigD: sigma factor, PubMed, in sigD regulon

• Genes: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

  Regulatory mechanism

  • Spo0A: repression, in spo0A regulon
  • SwrA: activation, in swrA regulon
  • CodY: repression, in codY regulon
  • DegU: repression, in degU regulon
  • SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• DS7306 (marker-less in NCIB3610) PubMed
• BKE16420 (ΔcheB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAACTACAAGCACACGAA,  downstream forward: _UP4_AAAAAAGAGAGGGTGTGATT
• BKK16420 (ΔcheB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCAACTACAAGCACACGAA,  downstream forward: _UP4_AAAAAAGAGAGGGTGTGATT

Labs working on this gene/protein

• Daniel Kearns

References

Reviews

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Original Publications

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