Difference between revisions of "CheY"

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(Expression and regulation)
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}}
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Mutant:'''
 
* '''Mutant:'''
 
** 1A798 ( ''cheY''::''cat''), {{PubMed|1905718}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A798&Search=1A798 BGSC]
 
** 1A798 ( ''cheY''::''cat''), {{PubMed|1905718}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A798&Search=1A798 BGSC]
 +
** DS6870 (marker-less in NCIB3610) {{PubMed|25313396}}
 +
** TB191 ''amyE''::Phy-''sfgfp'' (marker-less in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}}
 +
** TB207 ''amyE''::Phy-''mKATE2'' (marker-less in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}}
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
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=References=
 
=References=
 
==Reviews==
 
==Reviews==
<pubmed>19494578,,10094672 20122866  16224120 </pubmed>
+
<pubmed>25313396, 26122431, 19494578,,10094672 20122866  16224120 </pubmed>
  
 
==Original Publications==
 
==Original Publications==

Revision as of 13:01, 2 July 2015

  • Description: two-component response regulator, modulation of flagellar switch bias

Gene name cheY
Synonyms cheB
Essential no
Product two-component response regulator
Function modulation of flagellar switch bias
Gene expression levels in SubtiExpress: cheY
Interactions involving this protein in SubtInteract: CheY
MW, pI 13 kDa, 4.746
Gene length, protein length 360 bp, 120 aa
Immediate neighbours fliY, fliZ
Sequences Protein DNA DNA_with_flanks
Genetic context
CheY context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheY expression.png















Categories containing this gene/protein

transcription factors and their control, motility and chemotaxis, phosphoproteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16330

Phenotypes of a mutant

  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YneI

Extended information on the protein

  • Kinetic information:
  • Modification:
    • phosphorylated by CheA on an Asp residue
    • dephosphorylated by CheC PubMed
  • Effectors of protein activity:

Database entries

  • Structure: 1TMY (CheY from Thermotoga maritima)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • in minimal medium, CheY is present with 7,100 +/- 1,000 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1164 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 2117 PubMed

Biological materials

  • Mutant:
    • 1A798 ( cheY::cat), PubMed, available at BGSC
    • DS6870 (marker-less in NCIB3610) PubMed
    • TB191 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB207 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original Publications