Difference between revisions of "CheV"
| Line 54: | Line 54: | ||
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
* ''[[cheV]] [[cheW]]'' double mutants exhibit complete loss of chemotaxis {{PubMed|21098025}} | * ''[[cheV]] [[cheW]]'' double mutants exhibit complete loss of chemotaxis {{PubMed|21098025}} | ||
| + | * not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation {{PubMed|26122431}} | ||
| + | |||
=== Database entries === | === Database entries === | ||
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14010&redirect=T BSU14010] | * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU14010&redirect=T BSU14010] | ||
| Line 125: | Line 127: | ||
* '''Mutant:''' | * '''Mutant:''' | ||
| + | ** DS70 (mls in NCIB3610) {{PubMed|12864845}} | ||
| + | ** TB183 ''amyE''::Phy-''sfgfp'' (mls in NCIB3610 with constitutive expressed ''sfgfp'') {{PubMed|26122431}} | ||
| + | ** TB199 ''amyE''::Phy-''mKATE2'' (mls in NCIB3610 with constitutive expressed ''mKATE2'') {{PubMed|26122431}} | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| Line 144: | Line 149: | ||
<pubmed>18774298,</pubmed> | <pubmed>18774298,</pubmed> | ||
==Original publications== | ==Original publications== | ||
| − | <pubmed>8169224,8169223,11553614,14731274,21098025, 21515776</pubmed> | + | <pubmed> 12864845,26122431, 8169224,8169223,11553614,14731274,21098025, 21515776</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:58, 2 July 2015
- Description: modulation of CheA activity in response to attractants
| Gene name | cheV |
| Synonyms | |
| Essential | no |
| Product | CheA modulator |
| Function | control of CheA activity |
| Gene expression levels in SubtiExpress: cheV | |
| Interactions involving this protein in SubtInteract: CheV | |
| MW, pI | 34 kDa, 4.617 |
| Gene length, protein length | 909 bp, 303 aa |
| Immediate neighbours | ykzT, kre |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
transcription factors and their control, phosphoproteins, motility and chemotaxis
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14010
Phenotypes of a mutant
- cheV cheW double mutants exhibit complete loss of chemotaxis PubMed
- not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed
Database entries
- BsubCyc: BSU14010
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s): CheW (N-terminal domain of CheV)
Extended information on the protein
- Kinetic information:
- Modification: the C-terminal two-component receiver domain is phosphorylated on a Asp residue by CheA PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- forms lateral clusters (phosphorylated form), but in the presence of high asparagine concentration (non-phosphorylated form) there is a reversible re-localization to the poles of the cell PubMed
Database entries
- BsubCyc: BSU14010
- Structure:
- UniProt: P37599
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Reviews
Christopher V Rao, George D Glekas, George W Ordal
The three adaptation systems of Bacillus subtilis chemotaxis.
Trends Microbiol: 2008, 16(10);480-7
[PubMed:18774298]
[WorldCat.org]
[DOI]
(P p)
Original publications
Theresa Hölscher, Benjamin Bartels, Yu-Cheng Lin, Ramses Gallegos-Monterrosa, Alexa Price-Whelan, Roberto Kolter, Lars E P Dietrich, Ákos T Kovács
Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development.
J Mol Biol: 2015, 427(23);3695-3708
[PubMed:26122431]
[WorldCat.org]
[DOI]
(I p)
Vincent J Cannistraro, George D Glekas, Christopher V Rao, George W Ordal
Cellular stoichiometry of the chemotaxis proteins in Bacillus subtilis.
J Bacteriol: 2011, 193(13);3220-7
[PubMed:21515776]
[WorldCat.org]
[DOI]
(I p)
Kang Wu, Hanna E Walukiewicz, George D Glekas, George W Ordal, Christopher V Rao
Attractant binding induces distinct structural changes to the polar and lateral signaling clusters in Bacillus subtilis chemotaxis.
J Biol Chem: 2011, 286(4);2587-95
[PubMed:21098025]
[WorldCat.org]
[DOI]
(I p)
Michael W Bunn, George W Ordal
Receptor conformational changes enhance methylesterase activity during chemotaxis by Bacillus subtilis.
Mol Microbiol: 2004, 51(3);721-8
[PubMed:14731274]
[WorldCat.org]
[DOI]
(P p)
Daniel B Kearns, Richard Losick
Swarming motility in undomesticated Bacillus subtilis.
Mol Microbiol: 2003, 49(3);581-90
[PubMed:12864845]
[WorldCat.org]
[DOI]
(P p)
E Karatan, M M Saulmon, M W Bunn, G W Ordal
Phosphorylation of the response regulator CheV is required for adaptation to attractants during Bacillus subtilis chemotaxis.
J Biol Chem: 2001, 276(47);43618-26
[PubMed:11553614]
[WorldCat.org]
[DOI]
(P p)
M M Rosario, K L Fredrick, G W Ordal, J D Helmann
Chemotaxis in Bacillus subtilis requires either of two functionally redundant CheW homologs.
J Bacteriol: 1994, 176(9);2736-9
[PubMed:8169224]
[WorldCat.org]
[DOI]
(P p)
K L Fredrick, J D Helmann
Dual chemotaxis signaling pathways in Bacillus subtilis: a sigma D-dependent gene encodes a novel protein with both CheW and CheY homologous domains.
J Bacteriol: 1994, 176(9);2727-35
[PubMed:8169223]
[WorldCat.org]
[DOI]
(P p)
