cotA

168

laccase, bilirubin oxidase, spore coat protein (outer)

Locus: BSU_06300

Molecular weight: 58.33 kDa

Isoelectric point: 5.89

Protein length: 513 aa

Gene length: 1542 bp

Function: resistance of the spore

Product: laccase, bilirubin oxidase

Essential: no

E.C.: 1.3.3.5

Synonyms: cotA, pig

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2132 (Galperin et al., 2021)

Gene

Coordinates: 683,462  685,003

The protein

Protein family

• S.antibioticus phenoxazinone synthase (PhsA)

Cofactors

• Cu(I)

Structure

• 2BHF (PDB) (reduced form)
• P07788 (AlphaFold)

Localization

• outer spore coat, more abundant at the mother cell-distal pole of the forespore, localization depends on CotE  PubMed

Expression and Regulation

Operons

• Genes: cotA

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: repression, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

Mutant

• 1S101 ( cotA::cat), PubMed, available at BGSC
• 1S134 ( cotA::kan), PubMed, available at BGSC
• BKE06300 (cotA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCATCTGTCCTTATC,  downstream forward: _UP4_TAACCCGACAAACTTGCCTC
• BKK06300 (cotA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCATCTGTCCTTATC,  downstream forward: _UP4_TAACCCGACAAACTTGCCTC

References

Reviews

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Original Publications

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