Difference between revisions of "YabT"

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(References)
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* '''Description:''' [[protein kinases and phosphatases|Ser/Thr kinase]], controls DNA integrity during spore development <br/><br/>
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* '''Description:''' [[protein kinases and phosphatases|Ser/Thr kinase]], controls [[translation]] and DNA integrity during spore development <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
** ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
 
 
** phosphorylation of [[RecA]] on Ser-2 {{PubMed|23634894}}
 
** phosphorylation of [[RecA]] on Ser-2 {{PubMed|23634894}}
 +
** phosphorylation of [[EF-Tu|tufA]] on Thr-63 during [[sporulation]] {{PubMed|26056311}}
  
 
* '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot)
 
* '''Protein family:''' [[protein kinases and phosphatases|protein kinase]] domain (according to Swiss-Prot)
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** [[YabT]]-[[RecA]] {{PubMed|23634894}}
 
** [[YabT]]-[[RecA]] {{PubMed|23634894}}
 
** [[AbrB]]-[[YabT]] {{PubMed|24731262}}
 
** [[AbrB]]-[[YabT]] {{PubMed|24731262}}
 +
** [[AbrB]]-[[YabT]] {{PubMed|26056311}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
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=References=
 
=References=
  
<pubmed>16497325,23634894, 24731262 25278935 </pubmed>
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<pubmed>16497325,23634894, 24731262 25278935 26056311</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 14:19, 15 June 2015

Gene name yabT
Synonyms
Essential no
Product Ser/Thr kinase
Function control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI 37 kDa, 9.943
Gene length, protein length 1014 bp, 338 aa
Immediate neighbours yabS, tilS
Sequences Protein DNA DNA_with_flanks
Genetic context
YabT context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YabT expression.png















Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

  • Locus tag: BSU00660

Phenotypes of a mutant

  • increased sensitivity to DNA damage during spore development PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
    • autophosphorylation is stimulated by non-specific binding to DNA PubMed
  • Localization:
    • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation: expressed early during sporulation in the forespore (SigF) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
    • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab
  • lacZ fusion:
  • translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References